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. 2010 Sep 8;285(47):36456–36470. doi: 10.1074/jbc.M110.156588

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Soluble, uncleaved ΔV1V2 trimers can be expressed efficiently. A, SDS-PAGE analysis of uncleaved JR-FL SOSIP.R6-IZ-His gp140 variants expressed transiently in 293T cells. B, blue native-PAGE analysis of uncleaved JR-FL SOSIP.R6-IZ-His gp140 from two independent transfections (left and right panels). C, size exclusion analysis of gp140 trimers. Uncleaved JR-FL SOSIP.R6-IZ-His gp140 trimers produced in 293T cells were fractionated using a Superose-6 column. The fractions were analyzed by SDS-PAGE and Western blotting. The elution peaks of reference proteins are indicated.