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Altered pH sensitivity of SVCT2 histidine mutants expressed in HEK-293 cells. 48 h after transfection, the cells were incubated for 30 min at different pH values (6.0–9.0) and then subjected to a 5-min transport assay at the same pH. Transport data correspond to the means ± S.D. (error bars) of one experiment of two performed in triplicate. AA, ascorbic acid.
