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. 2010 Sep 15;285(47):36471–36485. doi: 10.1074/jbc.M110.155630

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Time course of the effect of DEPC on ascorbic acid (AA) transport by SVCT2 and histidine mutants H203Q and H413Q. 48 h after transfection, cells expressing SVCT2 (A), mutant H203Q (B), or mutant H413Q (C) were incubated with graded concentrations of DEPC and submitted to a 5-min transport assay using 100 μm ascorbic acid. D, concentration-response curve for the effect of DEPC on the rate of ascorbic acid transport after a 10-min modification experiment with DEPC. E, concentration-response curve for the effect of DEPC on the initial rate of decrease of the ascorbic acid transport rate. For each curve, the slope of the initial decrease in transport rate was obtained by linearization of the first 3 min of the time course curve of DEPC treatment. Transport data correspond to the means ± S.D. (error bars) of one experiment of two performed in triplicate.