TABLE 1.
Functional properties of SVCT2 histidine mutants
Values are from one experiment of two performed in triplicate. PM, plasma membrane.
| Mutant | Localization | Vmax | Km | nH | Na50+ |
|---|---|---|---|---|---|
| pmol/106 cells/min | μm | ||||
| SVCT2 | PM | 994 ± 34 | 24 ± 13 | 1.7 ± 0.1 | 29 ± 2 |
| H203Q | PM | 865 ± 34 | 28 ± 13 | 2.2 ± 0.2 | 33 ± 2 |
| H206Q | PM | 914 ± 34 | 28 ± 13 | 2.3 ± 0.3 | 22 ± 2 |
| H413Q | PM | 982 ± 11 | 68 ± 2 | 1.7 ± 0.2 | 20 ± 2 |
| H29Q | PM | 994 ± 34 | 24 ± 13 | 1.7 ± 0.1 | 34 ± 2 |
| H109Q | PM | ||||
| H269Q | PM | 1307 ± 24 | 95 ± 4 | 1.7 ± 0.1 | 20 ± 2 |
| H203Q/H206Q | PM | 986 ± 14 | 67 ± 3 | 1.6 ± 0.4 | 28 ± 2 |
| H203Q/H413Q | PM | 632 ± 21 | 28 ± 2 | 1.4 ± 0.1 | 26 ± 1 |
| H206Q/H413Q | PM | 994 ± 34 | 24 ± 13 | 1.7 ± 0.1 | 29 ± 2 |
| H203Q/H206Q/H413Q | Intracellular | 98.2 ± 2.4 | 23 ± 2 | 2.2 ± 0.2 | 34 ± 2 |