FIGURE 6.

Alterations in epididymal adipose tissue TAG content. A, two-dimensional electrospray ionization-MS profile of TAG molecular species from mice fed the standard diet. Adipose lipid extracts were analyzed by electrospray ionization-MS as described under “Experimental Procedures.” Spectra for WT (left) and iPLA₂γ^−/− (KO, right) epididymal adipose tissue TAG illustrate multiple alterations of individual TAG species. Panels are representative mass spectra from extracts of adipose tissue from at least three mice for each condition. Ion intensities for all samples were normalized to a T17:1 TAG internal standard (IS) for quantitation after correction (28). B, comparison of total epididymal TAG from WT and iPLA₂γ^−/− mice fed either the standard (Std) or the HF diet. Total TAG decreased by ∼15% (p = 0.06) in the KO on the standard diet (left) and 57% (p < 0.001) on the HF diet (right). Male mice 8–10 months of age were utilized in these studies. Data are presented as means ± S.E. and statistical significances are indicated by the p values above the brackets. WT, closed bars; KO, open bars. n = 3–4 per group.