TABLE 3.

qPCR analyses of intracellular phospholipases

Expression of patatin-like intracellular phospholipases in wild-type (WT) and iPLA₂γ^−/− (KO) epididymal adipose tissue from mice on the standard and HF diets. qPCR analyses were performed as described under “Experimental Procedures” utilizing three male mice per group (ages 6–8 months). PNPLA4 is not present in the mouse genome. The mean values are presented along with standard error, and p values were determined by the Student's t test.

Gene	Standard diet			HF diet
	WT	KO	p value	WT	KO	p value
PNPLA1	0.44 ± 0.24	0.40 ± 0.23	0.9	0.55 ± 0.14	3.40 ± 1.10	0.004
PNPLA2	11.0 ± 0.08	2.0 ± 0.16	0.01	1.20 ± 0.21	0.90 ± 0.14	0.05
PNPLA3	6.5 ± 0.56	1.2 ± 0.34	0.001	1.42 ± 0.31	0.24 ± 0.08	0.001
PNPLA5	0.14 ± 0.07	0.07 ± 0.02	0.48	0.34 ± 0.03	0.48 ± 0.19	0.61
PNPLA6	4.8 ± 2.0	3.2 ± 0.7	0.2	0.74 ± 0.03	0.08 ± 0.28	0.57
PNPLA7	0.8 ± 0.06	1.3 ± 0.15	0.04	0.97 ± 0.03	0.83 ± 0.31	0.21
PNPLA9	0.77 ± 0.14	1.30 ± 0.12	0.02	1.45 ± 0.14	0.80 ± 0.12	0.02