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Pf-actin purification. Pf-actin was extracted from merozoites and then polymerized by adding actin polymerization buffer and 0–20 μm F-actin-stabilizing toxins phalloidin or jasplakinolide at pH 8.0. At pH 6.0 more actin could be polymerized in the presence of 20 μm phalloidin. Following centrifugation, soluble (monomeric actin) and the insoluble fractions (F-actin) were analyzed by SDS-PAGE and Western blotting to detect actin.
