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. 2010 Sep 7;285(47):36577–36585. doi: 10.1074/jbc.M110.142638

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — In vitro motility assay. a, Pf-actin filaments polymerized in the presence of 20 μm rhodamine-phalloidin at pH 8.0 were further incubated with unlabeled RS-actin. This produced long filaments of “zebra-striped” appearance with segments of fluorescent Pf-actin and unlabeled RS-actin. The hybrid filaments moved on a surface coated with skeletal muscle HMM (see supplemental movie S1). b, Pf-actin polymerized in the presence of 20 μm rhodamine-phalloidin at pH 6.0, and c, distribution of filament length of Pf-actin polymerized as in b.