FIGURE 5.

Loop 2 of the TLR3 ectodomain contributes to secretion. A, shown are sequences in Loop 2 affected in various mutants. The WT Loop 2 sequence is in bold, and the deleted residues are shown as dashes. Amino acid substitutions are underlined. B, shown are the effects of mutations in Loop 2 of T3ECD on secretion. Each construct was tested in two independently transfected cultures. In this assay, the medium and the cell lysates were loaded in the same well of the SDS-PAGE because the two bands can be clearly distinguished. The secreted (S) and cell-associated (C) T3ECD are identified to the right of the gel images. M denotes the molecular mass marker, and Vec. denotes that the cells were transfected with an empty vector. FL, full-length.