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. 2010 Sep 10;285(47):36689–36697. doi: 10.1074/jbc.M110.152132

FIGURE 8.

FIGURE 8.

Interactions between ARA7 and mutants of VPS9a. a, mutations in the conserved Asp185 and Tyr225 of VPS9a strongly affect the interaction between ARA7 and VPS9a. Note that the D185N mutation of VPS9a does not completely abolish the interaction, which is consistent with the results shown in Fig. 4. The quantity of ARA7 protein contained in 10% of the yeast lysate used in each assay is shown as an ARA7 input. b, dissociation constants (KD) between ARA7 and VPS9a with 1 mm Mg2+ or 1 mm EDTA buffer.