FIGURE 9.

Nucleotide-dependent interactions between ARA7 and VPS9a. Pull-down and yeast two-hybrid assays indicated that VPS9a preferred the dominant negative (S24N) or the nucleotide-free (N123I) forms of ARA7 to the constitutively active (Q69L) form. The quantity of the ARA7 protein contained in 10% of the yeast lysate used in each pull-down assay is shown (ARA7 input). AD and BD were fused to the transcription activating domain and DNA binding domain, respectively, in the yeast two-hybrid assay.