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The Golgi localization of the ARFGAPs is dependent on ARFGTP. HeLa wild-type cells were treated with BFA (5 μg/ml) for 1 min and stained with antibodies toward ARFGAP1–3 (left). To verify that this treatment did not affect Golgi structure, cells were co-stained with antibody against β1,4-galactosyltransferase I (GalT; right). Scale bar, 10 μm.
