FIGURE 7.

Electron microscopy of cells transfected with RNAi^Mock and RNAi^ARFGAP2 and RNAi^ARFGAP3. Thin plastic embedded sections (60 nm thick) of HeLa cells were examined at the ultrastructural level. Knockdown of ARFGAP2 and ARFGAP3 caused significant structural impairment of the Golgi stacks. Top left, cells transfected with RNAi^Mock have aligned stacks that are part of the Golgi ribbon. Top right and bottom left, cells transfected with RNAi^ARFGAP2 and RNAi^ARFGAP3 show Golgi regions composed of fewer cisternae and stacked structures. Bottom right, quantitation of number of cisternae and stacked cisternae as compared with control (Mock). Error bars, S.E.