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. 2010 Sep 21;285(47):36709–36720. doi: 10.1074/jbc.M110.180380

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — The ARFGAP2 and ARFGAP3 pair is essential for generation of the coat lattice. Cells treated with siRNA against the ARFGAPs or combinations thereof were treated with AlF or BFA or double-treated with AlF and BFA. Treated cells were stained for coatomer. Cells treated with control or siRNA against ARFGAP1 demonstrate BFA resistance after AlF treatment. After double knockdown (KD) of ARFGAP2 and ARFGAP3, coatomer becomes sensitive to BFA with redistribution of coatomer to the cytosol. Scale bar, 11 μm.