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. 2010 Sep 17;285(47):36759–36767. doi: 10.1074/jbc.M110.166231

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — FXR deficiency alters triglyceride storage, lipolysis, de novo lipogenesis, and the expression of lipid droplet genes in MEFs during differentiation to adipocytes. A, TG content in FXR^−/− and FXR^+/+ MEFs at days 0, 4, and 8 of differentiation treated with 1 μm rosiglitazone; lipolysis was measured in FXR^−/− and FXR^+/+ MEFs at days 0, 4, and 8 as glycerol release under basal and stimulated (isoproterenol: ISO) conditions. De novo lipogenesis in FXR^−/− and FXR^+/+ MEFs was measured at days 0, 4, and 8 of differentiation. The results are representative of three experiments and are presented as means ± S.D. B, quantification of the lipid droplet size of 8-day-differentiated FXR^−/− and FXR ^+/+ MEFs. C, mRNA expression of genes coding for lipid droplet proteins in FXR^−/− and FXR^+/+ MEFs during differentiation measured by quantitative PCR. Values are normalized to the expression of cyclophilin and are expressed relative to those at day 0, which are arbitrarily set to 1. The results are representative of three experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.