FIGURE 2.
Agarose gel electrophoresis of phage P22 and its ejection products. A, EtBr stained 1% agarose gel electrophoresis with 1.75 × 109 complete P22 phages (lane 1) or complete phage particles with 10 μg/ml DNase (lane 2), 1.75 × 109 phage particles incubated with LPS over night (lane 3), and thereafter with 10 μg/ml DNase (lane 4). B, electrophoretic analysis of DNA released from intact phage particles upon incubation with LPS (lanes 1, 3, and 5) and of phage DNA purified from denatured particles (lanes 2, 4, and 6), either untreated (lanes 1 and 2) or digested with AvaI (lanes 3 and 4) or ClaI (lanes 5 and 6).