FIGURE 4.
Glucose uptake test in vivo and in vitro. A, 2-deoxyglucose-uptake experiment in vivo without injecting insulin. Slightly increased glucose uptake was observed in all nine tissues in Sirt6Δ2–3/Δ2–3 mice compared with wild-type control mice. Five pairs of P25–26 mice at fed condition were used in the assay. B, insulin levels of the mice used in the 2-DG uptake test in A. C, 2-deoxyglucose-uptake experiment conducted 40 min. after injection of insulin (0.75 units/kg body weight). Significantly increased glucose uptake was observed in most organs/tissues examined in Sirt6Δ2–3/Δ2–3 mice compared with wild-type control mice. At least five pairs of fed mice at P25–28 were used in the assay. D, 2-deoxyglucose-uptake conducted in C2C12 cells that were infected with lentiviral shRNA specific to SIRT6 or to luciferase. (SIRT6 knockdown efficiency was similar to that shown in Fig. 5E.) E, 2-deoxyglucose-uptake conducted in SIRT6 mutant and control MEFs. * represents p < 0.05 by Student's t test.