TABLE 2.

Effect of glucose or amino acids on pyridine nucleotide levels in dksA mutant Salmonella

Data are from 12–16 independent observations recorded in at least four separate experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. The p values in the control group are comparisons between wild-type (WT) and dksA mutant. The p values in the glucose and casamino acids groups are comparisons with the corresponding PBS control.

Nucleotide pool	Control		Glucose		Casamino acids
	WT	dksA	WT	dksA	WT	dksA
NADH	90 ± 21a	49 ± 8*	228 ± 8***	243 ± 35***	234 ± 46**	250 ± 92**
NAD+	690 ± 195	1089 ± 218**	942 ± 121	971 ± 122	710 ± 79	1111 ± 155
NADH/NAD+	0.130	0.045	0.242	0.250	0.330	0.225
NADPH	410 ± 89	310 ± 80**	446 ± 114	815 ± 228**	620 ± 43**	707 ± 103**
NADP+	1026 ± 87	1390 ± 254*	485 ± 87	570 ± 148**	456 ± 97***	567 ± 90***
NADPH/NADP+	0.399	0.223	0.922	1.430	1.361	1.248

^a μm/cell.