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. 2010 Sep 21;285(47):36828–36835. doi: 10.1074/jbc.M110.157792

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Msc1 associates with Swr1, and its stability is compromised in cells lacking Swr1. A, Msc1 co-immunoprecipitated with Swr1. NW2632 cells with HA-tagged Msc1 and FLAG-tagged Swr1, each at their respective chromosomal locus, were grown to mid-log phase, lysed, and immunoprecipitated (IP) with antibody (Ab) to either the HA or the FLAG epitope. Captured immune complexes were separated by SDS-PAGE and subjected to Western blot analysis with antibody to HA or FLAG. B, cells with endogenous HA-tagged Msc1 and either wild-type (NW1562) or deleted (NW2633) Swr1 were grown to mid-log phase, fixed, and incubated with antibody to the HA epitope. Cells were stained with secondary antibody coupled to Cy3 and with DAPI to visualize nuclei. C, lysates from mid-log phase cells with (NW1562) or without (NW2633) Swr1 were loaded as serial dilutions, separated by SDS-PAGE, transferred to nitrocellulose, and probed with antibody to detect the HA epitope on Msc1.