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Histone H2A.Z localization to the otr1 region of centromere I is dependent on Swr1 but not on Msc1. Cells with an integrated copy of HA-tagged pht1 (NW1776) and HA-tagged pht1 with deletion of either msc1 (NW1959) or swr1 (NW2048) were grown to mid-log phase and processed as described under “Experimental Procedures” for chromatin immunoprecipitation. Input and precipitated samples were probed with primers for the cnt1, imr1, and otr1 regions of the centromere and for the fbp1 gene.
