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. 2010 Sep 10;285(47):36922–36932. doi: 10.1074/jbc.M110.126714

FIGURE 4.

FIGURE 4.

KDM6B is targeted by miR-148a, miR-185, and miR-339-5p. Panels A–C represent single pre-miRNAs, combinations of two pre-miRNAs, and combinations of three or all four pre-miRNAs, transfected into ND7 cells. Two negative controls, a no pre-miRNA transfection control and a non-targeting negative control miRNA from Ambion, were used. GAPDH was the protein loading control. Experiments were performed in quadruplicate with each data point in duplicate. Single miRNAs did not silence KDM6B (A). Combinations of two miRNAs that were most effective in silencing KDM6B were miR-339-5p + miR-185 and miR-339–5p + miR-148a (B). For >90% silencing of KDM6B, combinations of at least 3 miRNAs were necessary: miR-339-5p (red dot) + miR-185 (red dot) plus either miR-148a (green dot) or miR-138 (green dot) (C). Experiments were performed in quadruplicate with each data point in duplicate.