FIGURE 7.
Quantitative real time RT-PCR of Hes1 and Neurog2 from E10.5 embryos. Expression of murine Hes1 and Neurog2 was analyzed by RT-PCR. The data (n = 4; mean ± S.E.) shows ΔΔCt values for Hes1 and Neurog2 transcript levels in Sp−/− and FA-rescued Sp−/− embryos compared with WT littermates. Data were normalized to β-actin. ΔΔCt values are obtained by subtracting the Ct value for the WT gene from Sp−/− and FA-rescued Sp−/− embryos after normalization. Experiments were performed in quadruplicate with each data point in duplicate.