FIGURE 8.

ChIP assays showing chromatin remodeling on Hes1 and Neurog2 promoters. Individual ChIP assays were performed using neurospheres from caudal neural tube portions of WT, Sp^−/−, and Sp^−/−-treated with FA (E10.5 and E12.5). IgG was used as an IP negative control. β-Actin primers were used as negative controls. Amplified product was present only in the input and not in the control IgG or the immunoprecipitate. Immunoprecipitated DNA was subjected to quantitative PCR using murine primers for Hes1 (A) and Neurog2 (B) promoters. The data represents fold-enrichment of immunoprecipitated DNA compared with the input sample. FA rescued chromatin marks on Hes1 at E10.5, and on Neurog2 at E12.5. Each ChIP experiment was performed in triplicate using one lumbar neural tube region per ChIP assay with a total n = 4.