FIGURE 6.

Prefibrillar and fibrillar Aβ oligomers as well as soluble Aβ are not changed by the deletion of apoA-I in APP/PS1 mice. Soluble proteins were extracted from the cortices and hippocampi of APP/PS1/WT and APP/PS1/KO mice using TBS-based tissue homogenizing buffer as described under “Experimental Procedures.” A, prefibrillar Aβ oligomers were examined by dot blotting using A11 conformation-specific antibody. B, fibrillar Aβ oligomers were examined by dot blotting using OC conformation-specific antibody. In A and B, dot blot with 6E10 was performed and used to normalize the results of A11 and OC immunoreactivity. Shown is an ELISA for soluble Aβ₄₀ (C) and Aβ₄₂ (D). Note that Aβ₄₀, unlike Aβ₄₂, is significantly increased in APP/PS1/KO mice. Results are from 11–13 mice/group. Error bars, S.E.