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ITC of FnZ binding to Fn domains. The binding isotherms for FnZ(370–428) with the 100-kDa fragment (A) and the interaction of FnZ peptide 370–391 with GBD (B) and 8F19F1 (C) are shown. The top panels show the heat differences upon injection of peptide, and the lower panels show the integrated heats of injection, with the best fit to a one-site binding model using MicroCal Origin. Thermodynamic parameters for these experiments are summarized in Table 1.
