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Competitive binding of FnZ peptide to 8F19F1 in presence of collagen peptide, demonstrated by fluorescence anisotropy measurements. Unlabeled FnZ peptide at increasing concentrations was incubated with unlabeled 8F19F1 at 10 (■), 20 (○), and 30 μm (♦) and the collagen peptide labeled with 5-carboxyfluorescein. Data were fit using Origin software. Error bars (S.D.) correspond to instrumental readout error and derive from triplicate measurements.
