FIGURE 6.

Endocytic cargo protein Tat2-EGFP did not colocalize with autophagy-related structures in both pep4Δ and ESCRT mutant cells. A and B, fluorescence microscopic analyses of Tat2-EGFP and RFP-Atg8 localization in pep4Δ (A) and pep4Δvps27Δ (B) cells. Localization of Tat2-EGFP and RFP-Atg8 fluorescence was examined as in Fig. 5, B and D. Percentages noted below the images represent frequencies of the indicated phenotypes. Bar, 5 μm. DIC, differential interference contrast; NS, nitrogen starvation.