FIGURE 7.

ESCRT machinery is required for nitrogen starvation-triggered but not rapamycin-mediated autophagy. A, effect of ESCRT deficiency on nitrogen starvation-triggered GFP-Atg8 processing. Logarithmically growing WT and vps27Δ cells harboring pRS316-GFP-ATG8 were starved of nitrogen (NS). Total cell lysates were prepared at the indicated times and analyzed by Western blotting as in Fig. 3E. Asterisks indicate nonspecific signals. B, no effect due to ESCRT deficiency in rapamycin-triggered GFP-Atg8 processing. Logarithmically growing WT and vps27Δ cells harboring pRS316-GFP-ATG8 were treated with 20 ng/ml rapamycin. Expressions of GFP-Atg8 and cleaved GFP were analyzed by Western blotting as described in Fig. 3E.