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. 2010 Sep 7;285(47):36995–37004. doi: 10.1074/jbc.M110.133744

FIGURE 4.

FIGURE 4.

In vitro differentiation of C666-1 spheres. A, microscopic observation of C666-1 cells cultured as nonadherent undifferentiated spheres and adherent cells under differentiation conditions for 36 and 72 h. B, flow cytometry analysis of CD133+ (upper) and Oct-4-positive (lower) cell populations in C666-1 spheres and sphere-derived adherent progeny at 36 and 72 h. C, quantitative real time PCR analysis of miR-200a, CTNNB1, and ZEB2 expressions. mRNA levels in sphere were normalized as 1. PE, phycoerythrin.