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. 2010 Sep 7;285(47):36995–37004. doi: 10.1074/jbc.M110.133744

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Differential regulation of EMST by ZEB and CTTNB1 down-regulation in C666-1 cells. A, RT-PCR and Western blotting (WB) analysis of CTNNB1 and ZEB2 levels in siControl-, siCTNNB1-, and siZEB2-transfected C666-1 cells at 48 h post-transfection. GAPDH was shown as an internal control. B, phase contrast images of C666-1 cells after transient knockdown of CTNNB1 and ZEB2. C, Western blot analysis of epithelial (E-cadherin) and mesenchymal (vimentin) markers. D, flow cytometry analysis of CD133⁺ cell population; E, sphere formation assay in siControl-, siCTNNB1-, and siZEB2-transfected C666-1 cells. *, p < 0.05, as compared with siControl group.