FIGURE 1.

Cell migration of U87-FABP7(−) and U87-FABP7(+) cells in the presence of fatty acids. A, cells cultured in DMEM plus 10% FCS (growth medium) were treated with 60 μm BSA, 60 μm DHA, 60 μm AA, or 60 μm PA for 24 h. B, cells were serum-starved for 24 h and then treated with either BSA or the indicated fatty acids for 24 h. C, cells were cultured in growth medium and treated with different ratios of DHA and AA as follows: 1 = 30 μm; 2, = 60 μm; 3 = 90 μm; 4 = 120 μm. A–C, cells were trypsinized, and 20,000 cells (in DMEM) were plated in the upper chambers of Transwell inserts. The bottom chambers contained DMEM plus 10% FCS. After 6 h, the cells migrating through the porous membrane were fixed, stained, and counted using Metamorph imaging software version 7.7. Statistical significance was determined using the unpaired t test. Error bars represent standard deviation.