FIGURE 10.

COX-2 involvement in FABP7-induced cell migration. A, Western blot analysis of whole cell lysates (25 μg/lane) prepared from U87 cells stably transfected with FABP7 or empty vector. Lysates were electrophoresed in a 13.5% SDS-polyacrylamide gel, and the proteins were transferred to nitrocellulose membranes. Membranes were sequentially immunostained with goat anti-COX-2 antibody and mouse anti-actin antibody. B, ELISA was used to measure PGE₂ levels in U87-FABP7(−) versus U87-FABP(+) cell populations. The data were obtained from two independent experiments measured in triplicate. No error bars are shown for U87-FABP7(+) as all the wells were saturated for PGE₂. C, U87-FABP7(+) stable transfectants were treated 60 μm AA, 60 μm DHA, and/or 200 μm NS398, as indicated. Cell migration was measured using the Transwell assay as described previously. Statistical significance was determined using the unpaired t test (*, p < 0.01). Error bars represent standard deviation.