FIGURE 6.
FABP7 levels in U87MG cells transfected with wild-type and mutant FABP7 expression constructs. U87MG cells were transfected with pcDNA3.1-FABP7WT, -FABP7NLS, and -FABP7FAB expression constructs. Cytoplasmic (C) and nuclear (N) fractions were prepared, and 25 μg of protein was loaded in each lane. Proteins were electrophoresed in a 13.5% SDS-polyacrylamide gel, transferred to nitrocellulose membranes, and immunostained with anti-FABP7 antibody. The signal was detected using ECL reagent (GE Healthcare.