FIGURE 1.
W6/32 coimmunoprecipitates soluble U21NSBPHA-tagged molecules. a, hypothetical schematic depiction of U21 and class I MHC binding and depiction of the soluble U21NSBPHA molecule. b, U373, U21, and U21NSBPHA cells were labeled with [35S]methionine for 90 min, lysed, and recovered with W6/32, directed against properly folded class I MHC molecules. Migration positions of U21, U21NSBPHA, and class I heavy chain (HC) molecules are indicated. Note that this figure depicts a hypothetical interaction between U21 and class I molecules; interaction domains, if any, are unknown. IP, immunoprecipitation.