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Class I MHC molecules in U21-KDEL cells are retained in the ER. Top panels, control U373 cells, U21-, U21NSBPHA-, and U21NSBPHA-KDEL-expressing cells were immunolabeled with W6/32 to examine class I localization. Bottom panels, for comparison, these cells were labeled with anti-protein-disulfide isomerase (PDI) as a marker for ER immunolabeling. Ab, antibody.
