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. 2010 Sep 15;285(47):37041–37050. doi: 10.1074/jbc.M110.157115

FIGURE 4.

FIGURE 4.

Effect of 1α,25(OH)2D3, PLAA and AA on PKC activity and effect of 1α,25(OH) 2D3 on PGE2 release in wild type, Sh-Pdia3, and Ov-Pdia3 MC3T3-E1 cells. a, the effect of 1α,25(OH)2D3 on PKC activity of WT, Sh-Pdia3, and Ov-Pdia3 MC3T3-E1 cells. MC3T3-E1 cells were treated with vehicle (ethanol) or 10−10, 10−9, or 10−8 m 1α,25(OH)2D3 for 9 min. PKC activity was normalized to total protein. b, 1α,25(OH)2D3 effect on PGE2 release of WT, Sh-Pdia3, and Ov-Pdia3 MC3T3-E1. MC3T3-E1 cells were treated with vehicle (ethanol) or 10−10, 10−9, or 10−8 m 1α,25(OH)2D3 for 30 min. PGE2 in conditioned media was measured and normalized to cell number. c, PLAA effect on PKC activity of WT, Sh-Pdia3, and Ov-Pdia3 MC3T3-E1 cells. MC3T3-E1 cells were treated with vehicle (ddH2O) or 10−6, 10−7, or 10−8 m PLAA for 9 min. PKC activity was measured and normalized to total protein. d, AA effect on PKC activity of WT, Sh-Pdia3, and Ov-Pdia3 MC3T3-E1 cells. MC3T3-E1 cells were treated with vehicle (media) or 10−4, 10−5, or 10−6 m AA for 9 min. PKC activity was measured and normalized by total protein. *, p < 0.05, treatment versus control; ●, p < 0.05, 10−8 and 10−9 versus 10−10; #, p < 0.05 Sh-Pdia3 and Ov-Pdia3 versus WT for the same treatment.