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. 2010 Sep 16;285(47):37092–37101. doi: 10.1074/jbc.M110.174763

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Cellular localization and levels of Fkh1, Fkh2, Hcm1, and Fhl1. A, exponentially growing cells in YPD medium and carrying a chromosomically integrated FKH1-GFP (MJRC01), FKH2-GFP (MJRC02), FHL1-GFP (MJRC04), and HCM1-GFP (MJCR03) fusion were analyzed for GFP fluorescence. Nuclear co-localization was analyzed using DAPI staining. GFP and DAPI staining was analyzed with fluorescence microscopy. B, exponentially growing cells in YPD medium and carrying an integrated HA tag, FKH1–3HA (GRB2409), FKH2–3HA (GRB2411), HCM1–3HA (GRB2405), and FHL1–3HA (GRB2408) were analyzed by Western blot with anti-HA antibodies. Bands corresponding to each FKH-TF were measured with a densitometer, and the relative intensities were calculated. The data are represented as the means ± S.D. from three independent experiments.