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Hcm1 localization shifts from nucleus to the cytoplasm according to its cell cycle regulation. A, HCM1-GFP cells grown exponentially in YPD were treated with α-factor for 2 h. Cells arrested in G1 were released from α-factor, and pictures were taken at different time points by fluorescence microscopy. B, percentage of cells showing nuclear or cytoplasmic localization was quantified. Black bars, nuclear; gray bars, cytoplasmic.
