FIGURE 7.

Increased levels of Hcm1 produce a metabolic shift from glucose fermentation to respiration. Cultures of WT, Δhcm1, and tetHCM1 (minus and plus doxycycline, doxy) were grown in YPD medium. A, growth curves up to 40 h were measured. Duplication times were obtained at the exponential phase. The data are the averages of two independent experiments. B, alcohol dehydrogenase (ADH) activity was measured at the exponential phase. C, oxygen consumption (nmol O₂·min⁻¹) was measured by a Clark electrode at exponential phase. B and C, data are represented as the means ± S.D. from three independent experiments. D, amounts of three mitochondrial proteins (α-KGDH, Sod2, and porin) were assessed by immunoblot analysis at exponential phase. Actin was used as a loading control. E, levels of Abf2 were determined by immunoblot analysis at exponential phase. Actin was used as a loading control. The data are represented as the means ± S.D. from three independent experiments. Statistical analysis was performed comparing mutant and WT cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001.