Eosinophil peroxidase (EPO) activity in lung tissue 72 h after challenge. OVA-sensitized animals were challenged with either 400 μg MSA (OVAc) or OVA (OVA), and TMA-sensitized animals were challenged with either 400 μg MSA (TMAc) or TMA-MSA (TMA). (A) All sensitized and challenged animals. Values represent the geometric mean + SE for n = 18, 23, 28, and 21 animals for the four groups from left to right. (B) Subset of animals used for lung RNA isolation and microarray analysis. Values represent the geometric mean + SE for n = 8, 6, 6, and 7 animals for the 4 groups from left to right. NS = not significant. *p < 0.05. The lower set of brackets depicts the comparison of each allergen to its respective control. The upper bracket indicates the statistical comparison of the change in lung EPO (OVA-OVAc vs. TMA-TMAc). Geometric mean values are listed under respective treatment groups.