Table 1. Transport of amino acids by strains of R. leguminosarum bv. viciae.
Strain | Genotype | GlutamateVmax(Km) | AlanineVmax(Km) | AspartateVmax(Km) | LeucineVmax(Km) |
RU1721 | braEF::ΩTc | 9.80±0.30(106.3±11.8) | 10.44±0.15(215.5±89.7) | 6.75±0.75(75.7±12.4) | 4.71±0.14(391.7±76.49) |
RU1976 | aapQ P144D braEF::ΩTc | 2.42±0.28(36.0±3.02) | 5.32±0.76(91.0±1.0) | 3.22±0.50(53.0±3.7) | 1.37±0.17(279.0±80.5) |
RU1722 | aapJQM::ΩSp braEF::ΩTc | 0.2±0.1(ND) | 3.3±0.1(ND) | 0.2±0.1(ND) | 0.8±0.1(ND) |
Vmax and Km were calculated from transport rate mean values of 3 independent cultures measured at 5 concentrations from 0.1 µM up to 5 µM. Vmax values are given in nmol min−1mg−1 protein ± SEM and Km mean values are given in nM ± SEM. ND = not determined.