Table 6. Transport of amino acids by strains of Sinorhizobium meliloti.
Strain | Genotype | Glutamate | Isoleucine | Leucine | Valine | Alanine |
2011 | wild-type | 23.5±0.4 | 16.7±0.8 | 16.2 | 15.5 | 30.1 |
LMB210 | aapJ::ΩSpbraC::mTn5 | 0.8±0.1 | 12.0±0.6 | ND | ND | ND |
LMB211 | aapJ::ΩSpbraD::mTn5 | 0.6±0.1 | 0.7±0.3 | <0.4 | 0.8±0.2 | 4.4±0.4 |
All strains were grown in M9 modified glucose/NH4Cl minimal medium. Rates of uptake are expressed as nmol min−1mg−1 protein. Values are the mean ± SEM of determinations from three independent cultures, except for strain 2011 alanine, leucine and valine transports, which just served as controls and represent single measurements. ND = not determined.