Table 8. Strains, plasmids, primers.
| Strains, plasmids, primers | Description | Reference, source |
| Strain | ||
| 3841 | Rhizobium leguminosarum bv. viciae; Strr | [33] |
| RU1721 | braEF::ΩTc | This study |
| RU1976 | braEF::ΩTc aapQ P144D | This study |
| RU1722 | aapJQM::ΩSp braEF::ΩTc | [1] |
| 4292 | Sym plasmid pRL2JI (phaseoli) derivative of 8401 bv. phaseoli, Strr | [34] |
| RU2222 | 4292 with pLAFR1 | This study |
| RU1809 | aapJQM::ΩSp with pLAFR1 | This study |
| RU1932 | braE::TnphoA with pLAFR1 | This study |
| RU1933 | aapJQM::ΩSp braE::TnphoA with pLAFR1 | This study |
| 2011 | 2011, Strr derivative of Sinorhizobium meliloti | J. Dénarié, France |
| 2011mTn5STM.3.12.F11 | livK(braC)::mTn5 | [27] |
| 2011mTn5STM.4.05.D04 | livH(braD)::mTn5 | [27] |
| LMB210 | aapJ::ΩSp livK(braC)::mTn5 | This study |
| LMB211 | aapJ::ΩSp livH(braD)::mTn5 | This study |
| Plasmid | ||
| pLAFR1 | Wide-host-range mobilizable P-group cloning vector; Tcr | [35] |
| pJQ200SK | pACYC derivative, P15A origin of replication; Gmr | [36] |
| pPH1JI | P-group chaser plasmid | [37] |
| pRU1247 | aapJQMP region with AapQ P144D mutation on pJQ200SK | This study |
| pRU410 | aapJQM::ΩSp on pJQ200SK | [25] |
| pBIO206 | braE::TnphoA on pLAFR1 | [25] |
| pJET1.2 | Cloning vector | Fermentas, Lithuania |
| pLMB111 | Sm2011 2.6 kb aapJ fragment on pJET1.2 | This study |
| pLMB112 | aapJ deletion with BamHI site in pLMB111 | This study |
| pLMB113 | XbaI/XhoI fragment of pLMB112 cloned into pJQ200SK | This study |
| pLMB118 | ΩSp insertion into BamHI site of pLMB113 | This study |
| Primer | ||
| p495 | CACCACTAGTCATAGACCGAATCGACCAC | |
| p441 | AAACTAGTGGGTCTTTTCAAAGGATA | |
| p418 | GGGTCGCGAAACCAGACTTT | |
| p419 | CGCCGTACCACTTGTTCATG | |
| pIS50R | AGGTCACATGGAAGTCAGATC | |
| p439 | ATAGGGGCCGACACCGATGA | |
| pr0163 | GATTGCCGGAATATCCTGCA | |
| pr0164 | GCGGCAGACCGATGATCAGC | |
| pr0165 | TTTGGATCCTTTTTTGAACCTTTTCGGTT | |
| pr0166 | TTTGGATCCTGATCGGACGCTATGTCGGG | |
| pOTforward | CGGTTTACAAGCATAAAGC | |
| pr0240 | GCACGTGCGTCCCGCCGATC | |
| pr0241 | ACGATCTCGATCGACTGTCC |
Str = streptinomycin; Tc = tetracycline; Sp = spectinomycin; Gm = gentamicin; BamHI sites in primers are underlined.