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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2010 Oct 26;9(11):2893–2902. doi: 10.1158/1535-7163.MCT-10-0635

Figure 1. p53-independent PUMA induction by UCN-01 in colon cancer cells.

Figure 1

A. Chemical structure of UCN-01. B. WT and p53-knockout (p53-KO) HCT116 colon cancer cells were treated with increasing doses of UCN-01 for 24 hr. PUMA expression was analyzed by Western blotting. C. Left: p53-KO HCT116 cells were treated with 1 μM UCN-01 and mRNA extracted at indicated time points. Reverse transcription followed by real-time PCR was completed as described in Materials and Methods. Right: Following treatment of p53-KO HCT116 cells with 1 μM UCN-01, PUMA protein expression at the indicated times was analyzed by Western blotting. D. Five colon cancer cell lines with different p53 status were treated with UCN-01 for 24 hr. PUMA expression was analyzed by Western blotting.