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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2010 Oct 26;9(11):2893–2902. doi: 10.1158/1535-7163.MCT-10-0635

Figure 3. Induction of PUMA by UCN-01 is mediated through AKT inhibition.

Figure 3

A. p53-KO HCT116 cells were treated with 1 μM UCN-01 for 1 or 2 hr. Western blotting was used to analyze phospho-FoxO3a (p-FoxO3a), total FoxO3a, phospho-AKT (p-AKT), and total AKT. B. p53-KO cells were transfected for 24 hr with WT, constitutively active mutant AKT, or control empty vector. Indicated proteins were probed by Western blotting. C. p53-KO cells were transfected for 24 hr with constitutively active mutant AKT or control empty vector, and then were treated with 1 μM UCN-01 for 24 hr. PUMA and AKT expression were probed by Western blotting. D. p53-KO cells were treated with the AKT inhibitor triciribine (1 μM) or MK-2206 (25 μM) for 1 hr (for analysis of p-FoxO3a and p-AKT) or 24 hr (for analysis of PUMA), and indicated proteins were analyzed by Western blotting.