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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: J Microsc. 2009 Oct;236(1):52–59. doi: 10.1111/j.1365-2818.2009.03204.x

Figure 8.

Figure 8

An image of a rat tibial metaphysis, collected using serial milling and processed using the methods described in the current study, is shown. Two different fluorescent markers were applied to the animal prior to euthanasia, xylenol orange (shown in orange) followed one week later by calcein (shown in green). Three-dimensional images of the bone and the fluorescent markers of bone formation were collected using serial block face imaging with fluorescent markers processed using global thresholding. Future work will refine and validate the image processing techniques used to examine the fluorescent markers to allow direct, three-dimensional measures of eroded surfaces as well as the fluorescent markers of bone formation.