Fig. 1.

STAT1 and 3 are present in LV mitochondria. a Western blot analysis was performed for STAT1, 3, and 5 and marker proteins of subcellular compartments (Na⁺/K⁺-ATPase: sarcolemma, Serca2a: sarcoplasmic reticulum, HDAC2: nucleus, GAPDH: cytosol, ATP synthase α: mitochondria, VDAC: mitochondria) on rat total right ventricular (RV) and different amounts of LV SSM protein extracts (n = 4). Western blots were exposed to X-ray films such that similar immunoreactivities were obtained for STAT1, 3, and 5 in RV protein extracts. b Western blot analysis was performed for STAT1, 3, 5, and the mitochondrial marker protein VDAC on 25 μg total right ventricular (RV) and LV SSM proteins from WT and STAT3-KO mice (n = 4). c Western blot analysis was performed for STAT1 on SSM and IFM isolated from rat myocardium. Ponceau S staining is shown to demonstrate equal protein loading. The amount of STAT1 was normalized to Ponceau S staining of myocardial SSM and IFM. d Western blot analysis was performed for STAT3 on SSM and IFM isolated from rat myocardium. Ponceau S staining is shown to demonstrate equal protein loading. The amount of STAT3 was normalized to Ponceau S staining of myocardial SSM and IFM