Fig. 2.

Agrobacterium-mediated transformation in primary calli of Kasalath. a Rice calli induced on N6D medium from mature seeds of Nipponbare (left) and Kasalath (right) for 7 days. Bar 1 cm. b Transformed calli 21 days after the onset of selection on Hyg. Left panel, under blue light; right panel, under white light. c Regenerated shoots 24 days after the onset of regeneration. Left panel, under blue light; right panel, under white light. d Transgenic Kasalath plants in the T₀ generation. e An example of PCR analysis of the presence of the hpt gene in T₀ plants. The arrowhead indicates the 0.59 kb band corresponding to the hpt gene of the T-DNA. M DNA size marker, 1–20 genomic DNA extracted from transformed T₀ plants (lane 1–4 4 independent regenerated plantlets in Exp.3 shown in Table 1, lane 5–20 16 independent regenerated plantlets in Exp.4), G genomic DNA extracted from a non-transformant; N no template; V binary vector pCAMBIA1390-sGFP as template. f Southern blot analysis of 1.5 μg of EcoRI-digested genomic DNA extracted from T₀ plants using the gfp probe shown in Fig. 1. Lanes: NT non-transformant, 1–10 transgenic T₀ plants