Table 1.
Transformation efficiency of Kasalath and Nipponbare
| No. of calli infected with Agrobacterium (A) | No. of lines successful in clonal propagation (B) | No. of lines successfully regenerated (C) | Clonal propagation efficiency (B/A) (%)a | Regeneration efficiency (C/B) (%)b | |
|---|---|---|---|---|---|
| Kasalath-Exp.1 | 120 | 119 | 48 | 99.1 | 40.3 |
| Kasalath-Exp.2 | 124 | 121 | 81 | 97.6 | 66.9 |
| Kasalath-Exp.3 | 124 | 119 | 70 | 96.0 | 58.8 |
| Kasalath-Exp.4 | 121 | 118 | 65 | 97.5 | 55.1 |
| Nipponbarec | 96 | 88 | 80 | 91.7 | 91.0 |
aPrimary callus derived from one seed was counted as one line. Clonal propagation efficiency was calculated as the percentage of the number of clonal lines successfully propagated compared to the number of Agrobacterium-infected calli
bRegeneration efficiency was calculated as the ratio of regenerated plant lines in which T-DNA insertion was confirmed to the number of clonal lines successfully propagated. Significant difference between Nipponbare and Kasalath at P < 0.01, as determined by t test
cAs reported by Saika and Toki (2009b)