Table 2.
Independent transformation events from one primary callus
| No. of Hyg-resistant secondary callus lines observed on a single primary callus | No. of Hyg-resistant callus lines analyzeda | Average copy no. of T-DNAa | |
|---|---|---|---|
| Nipponbare-Exp.1 | 3 | 3/3 | 1.3 |
| Nipponbare-Exp.2 | 4 | 4/4 | 2 |
| Nipponbare-Exp.3 | 5 | 4/5 | 4.2 |
| Nipponbare-Exp.4 | 9 | n.d. | n.d. |
| Nipponbare-Exp.5 | 2 | n.d. | n.d. |
| Nipponbare-Exp.6 | 3 | n.d. | n.d. |
| Nipponbare-Exp.7 | 4 | 4/4 | 3.5 |
| Nipponbare-Exp.8 | 8 | 6/8 | 1.8 |
| Nipponbare-Exp.9 | 8 | n.d. | n.d. |
| Nipponbare-Exp.10 | 8 | n.d. | n.d. |
| Average | 5.4 ± 2.6b | 2.6 ± 2.1 | |
| Kasalath-Exp.1 | >50c | 8/8 | 2 |
| Kasalath-Exp.2 | 42 | 7/7 | 3.7 |
| Kasalath-Exp.3 | 41 | 8/8 | 2.1 |
| Kasalath-Exp.4 | >50c | n.d. | n.d. |
| Kasalath-Exp.5 | >50c | n.d. | n.d. |
| Average | 46.6 ± 4.7b | 2.6 ± 1.8 |
aIndependent transformation events and T-DNA copy numbers were determined by Southern blot analysis as shown in Fig. 5b
bSignificant difference between Nipponbare and Kasalath at P < 0.01, as determined by t test
cCalculated as 50